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Inverase was entrapped in calcium alginate gel. The immobilized beads had excellent uniform size and configuration. To screen the optimal conditions possessing high enzyme activity, effects of sodium alginate concentration, CaCl₂ concentration, and the incubation time of beads in CaCl₂ solution during the immobilization procedure were investigated. Immobilized beads prepared from the optimal conditions had 18.8 units per ㎖ of gel, which is equivalent to 68% of the activity of soluble enzyme. Several kinetic parameters were determined: Km value. 143 mM; optimum pH, 4.5; optimum temperature, 50℃. Enzyme loading capacity was 150 ㎎ per 20 ㎖ gel. No significant decrease in sugar conversion was observed during the column operation for 6 days.
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